Biological Sciences Symposia

(55) Low-voltage SEM Imaging of Lignocellulosic Biomass using a Low-cost Methanesulfonate Ionic Liquid

(56) Morphological study of PHA producing bacteria

(57) Obtention of phycobiliprotein nanoparticles from Spirulina (Arthrospira maxima) and its characterization by FTIR and microscopic techniques

(164) Optimizing Scanning Bessel Beam Light Sheet Microscopy with Custom-Designed Lens Cap for Expansion Microscopy

(165) Tissue Clearing of Whole-mount Alcian Blue and Eosin Stained Tissue to Investigate Cells Implicated in Regenerative Patterning

(166) Age and Hormonal Stimulation Affect Tyramine Enrichment and Smooth Muscle Modulation within the Male Mouse Reproductive System

(232) An automated approach to Synechocystis cell analysis in TEM image datasets

(233) Appraisal of AlphaFold2-Predicted Models in Cryo-EM Map Interpretation

(234) Automated Segmentation of Mitochondria in Virus-Infected Cells using Deep Learning Models

(237) High-Throughput Low-Dose Biomolecule Imaging in Liquid Phase Electron Microscopy

(238) Real-time Image Deblurring to Improve Throughput of Serial-Section Volume Electron Microscopy for Neural Connectomic Studies

(239) Using X-ray Microscopy and Machine Learning to Boost Image Quality in 3D Histology

(240) Accounting for the Ewald spheres in cryoEM reconstructions and their relationship to 3D Fourier transforms of focal series

(245) High-throughput, high-resolution data collection workflow for structure-based drug discovery using cryo-transmission electron microscopy

(246) Improving every image: HexAuFoil® ultra-small hole sample supports for CryoEM reconstructions.

(247) Maximize access to Cryo-EM learning and research tools with Web apps

(248) Measuring the effect of ice thickness and microscope configuration on resolution in single particle cryo-EM

(249) Microscope Operations at the National Center for CryoEM Access and Training (NCCAT)

(250) The National Center for CryoEM Access and Training - Establishing a cross-facility honored training curriculum

(251) VitroJet: Moving Sample Preparation into the New Era

(346) An Alternative Approach to Cryo-FIB Lift-out Using a Novel Cooled Nanomanipulator

(347) Automated Continuous Diffraction Tomography with Gatan Direct Detection Electron Counting Cameras: Advantages and Best Practices for Data Acquisition

(349) Fluorescence-guided cryo-lift-out using an integrated fluorescence light microscope and an optimized sample-needle attachment procedure

(350) High-resolution structure determination at 100kV enabled by new Falcon-C direct electron detector

(351) Improving Cryo-Electron Tomography Data Quality and Throughput by Streamlining the Workflow

(352) Precise 3D Localization by Integrated Fluorescence Microscopy (iFLM) for Cryo-FIB-milling and In-situ Cryo-ET

(353) Tomographic Particle Picking using 2D Single Particle Analysis Tools

(372) On the Electron Dose-rate Dependency of Radiation effects and Total Dose Criteria in ZIF-8 Metal-organic-framework (MOF)

(373) Preparation and Characterization of Carbon Dots Obtained from Different Low Molar Mass Precursors

(374) Structural Analysis of Chemically Cross-linked Carboxymethylcellulose Hydrogels added with Phytosynthesized Zinc Oxide Nanoparticles

(375) Surface Characterization on 5M NaOH Treated Ti-Ta Alloys Exposed to Simulate Body Fluid

(52) Different imaging techniques for the 2 and 3 D characterization of plant cell ultrastructure in the SEM and TEM

(53) Elemental physical and chemical analysis of PM10 by TEM-EDS

(54) Elimination of Human Error in Critical Point Drying Process in Plant Tissue Preparation for Electron Microscopy.

(154) Beauty Is in the AI of the Beholder

(163) Clearing and Whole Mount Immunohistochemistry for Smooth Muscle Actin Visualization during Regeneration

(167) Click Chemistry for Visualization of Newly Synthesized RNA and Antibody Labeling on Ultrathin Tissue Sections

(235) Data driven approach to delineate membrane structures in EM images using vesselness filter and machine learning model

(236) Expert- and Nonexpert-friendly Framework for Deep Learning Image Segmentation Demonstrates Successes Across Applications in vEM, CryoEM, MicroCT and Fluorescence Microscopy

(241) Chamelogator: A software tool for Chameleon data analysis

(242) Comparative Analysis of Cryo-Electron Microscopy and Liquid-Electron Microscopy Image Processing Workflows

(243) Cryo-EM Pipeline for Pharmaceutical and Biotechnology Industries

(244) CryoFAST™: Automated Cryo-Electron Microscopy Data Acquisition using Machine Learning methods

(254) Charting Cytoskeleton-Organelle Interplay in Living Cells Through High Resolution 3D Correlative Cryo-Imaging_2 Platform

(255) Dehydration: an alternative specimen preparation for soft x-ray tomography

(256) Development of Full Tilt Tomography in a Laboratory based Soft X-ray Microscope

(348) Cryo-Tomography of Cryo-EXLO Manipulated Yarrowia Lipolytica Yeast

(356) Compact GHz Ultrasonic Micro-Imager for Cells and Tissues

(357) Comparative characterisation of the expression profile of cardiac Kv7.1 channels containing two rare genetic variants

(358) Controlling the biochemistry on-the-fly and visualizing reaction dynamics during in-situ Liquid Phase TEM: A strong tool for biopharmaceutical development

(359) Development of a Cryo-Pre Embedding Immungold Labeling Protocol for the Ultrastructural Localization of PDL1 in Human Tonsils.

(360) Effects of Porosity and Stiffness of Fibroin-based Scaffolds on Osteoblast-like Cell Growth

(361) Electron Microscopists going MAD: Overcoming challenges in Mice, Ant, and Drosophila projects.

(370) Biofunctionalized gold nanoparticles obtained from two different plant extracts and its chemical and structural comparison

(371) Determining the Effect in the Adhesion Between Modified PLA Matrix and Natural Fibers Using SEM and Micro-Raman Spectroscopy

(353.1) 3D Electron Diffraction of Small Molecules on the MerlinEM Detector

(60) HDPE/Cherry Tree Fiber Composites: Size Particle Effect in the Flexural Mechanical Properties

(152) Annular Dark Field Imaging with variable angle for improving STEM tomography of Biological Samples

(169) An Integrated Solution for the Complete Serial Block-Face Scanning Electron Microscopy Workflow: From Image Acquisition to Data Processing

(61) Helical reconstruction of the giant bacteriophage AR9 tail at subnanometer resolution

(156) Image processing pipeline for in situ structural characterization of filaments

(363) In vitro Biofilm Formation by Bacillus subtilis and AR9 phage infection: SEM study

(367) Neuroprotection During Hypoxia Using Steroid Analogues

(366) Multi-faceted Return on Investment for Academic Centers and Cores

(365) Microstructural Characterization of Composites of Zinc Nitrate Crosslinked Carboxymethylcellulose Hydrogel and Biogenic Zinc Oxide Nanostructured

(65) The Ebola NP0VP35 complex phase separates into inclusion body-like structures, the disruption of which restricts viral infection

(160) STEM Tomography of Biological Samples Using Integrated Differential Phase Contrast Imaging Method

(162) 11-fold expansion microscopy with universal molecular retention using Magnify

(171) Method Development: Characterization of the Structure of the Thymic Epithelial Cell Network Utilizing Fluorescent Whole Slide Scanning and 3D SEM Array Tomography

(258) Soft X-ray tomography for mapping and quantifying intracellular organelle interactions

(354) Antioxidant Activity of Aqueous Extracts from Eucommia ulmoides and Cistanche deserticola: an in Vitro Study

(155) Highlights from the University of Virginia Molecular Electron Microscopy Core

(170) Array Tomography of mm-sized biosamples: impact of resin formulations on sample fidelity and image quality

(66) Understanding the roles of tcdE and tcdL during toxin secretion in Clostridioides difficile

(172) What Should I Do with My Serial Block-Face Data? Suggestions for Preparing, Analyzing, and Presenting Volume EM Datasets

(253) Analysis and Segmentation of Cytoplasm with U-Net_2 Poster

(369) Using Low kV STEM Imaging to Remove the Need for Post-Staining in Biological Sample Imaging

(161) Towards the Visual Proteomics of C. reinhardtii using High-throughput Collaborative in situ Cryo-ET

(252) A Laboratory-Based Soft X-ray Microscope for 3D Imaging of Whole Cells_2 Poster

(364) Micro- and Ultrastructure of the Yellow Grouper Epinephelus awoara Scale

(64) Structure of the Streptococcus pneumoniae 70S ribosome at 2.9 Å resolution using cryo-EM

(153) Assessing and Maximizing the Quality of 3DEM Structure Data at the Worldwide Protein Data Bank

(257) Soft X-ray 3D Imaging: A Powerful Tool for Visualizing Virus Infections with Increased Resolution and Field of View_2 Poster

(355) Application of Focused Ion Beam and Scanning Electron Microscopy for the Sectioning and Study of Acanthocephalan Hooks

(62) Molecular Structure of a Nodaviral Crown Complex

(157) Modern Tools for In-situ Tomography

(67) Workflow for High-resolution Sub-volume Averaging from Heterogenous Viral and Virus-like Assemblies

(158) Morphological Comparison of Primary Neurons Cryo-Preserved Under Varied Conditions

(362) Imaging the Cellular Distribution of Amino Acid Derivatives of Tricarbonylrhenium(I) 1, 10-orthophenanthroline Compounds

(63) New Morphologies of Hib Adhesion Pili

(159) Optimizing the protein stability in thick filament cryo-EM sample preparation using a PEGylation technique