X12 Short Course - Biological EM Sample Processing Short Course - Part Two

Course Organizers:
Ru-ching Hsia, Carnegie Institution for Science
Alice Liang, NYU Grossman School of Medicine
Kirk Czymmek, Donald Danforth Plant Science Center

This course is the second installment of a two-part series of biological EM course. The Part II course will focus on more advanced biological EM sample processing and techniques. It is recommended that students have prior experiences with Biological EM or possess knowledges of the principles and workflow of biological EM sample processing. Students who have taken the Part I course in 2022 are welcome to return. The lectures will cover the topics listed in the bullet point below:

Agenda:
8:30 – 9:00: Breakfast

9:00 – 10:00: Alternative fixation methods: Cryo and Microwave Methods - Kirk Czymmek & Ted Pella Inc.

10:00 – 11:00: Advanced Ultramicrotomy Techniques: Cryo-ultramicrotomy and Serial Sectioning Gnaegi Helmut

11:00 – 11:15: Break

11:15 – 12:15: Immuno Electron Microscopy Alice Liang

12:15 – 1:30: Lunch

1:30 – 2:30: Correlative Microscopy Kirk Czymmek

2:30 – 3:30: Practices of Image Analysis and Postprocessing, Segmentation and Visualization

3:30 – 4:00: Demo for Microwave - Ted Pella Inc.

4:00 – 5:00: Demo for Cryo-ultramicrotomy and Serial Sectioning Gnaegi Helmut